Study of enzyme kinetics for urease extracted from Glycine max

Abstract

Urease is an enzyme that has a key role in nitrogen metabolism in plant germination. Grains are produced more amount of urease than other plants. Glycine max is also called soya bean, a yearly vegetable of the pea family Fabaceae and it’s an eatable seed. Glycine max is mostly used as protein rich legume only, but it has a lot of medical and industrial applications too. Glycine max is used as a source of urease. The goal of this task is to study the kinetic activities of urease enzyme in Glycine max. The first enzyme was extracted from soya beans and partially purified. The enzyme activity was determined in both crude and partially purified using standard procedure. Further, enzyme kinetics (effect of temperature, pH, and substrate concentration) was studied using urease enzyme extracted from soya beans. The purification fold was 1.8 with a yield of 75%. The factors accustomed for its action was pH 8, temperature 50°C, Vmax and Km in Michaelis-Menten plot is 100 units and 8x10-4 moles/litre and LB Plot is 0.25units and 20x10-4 moles/litre.