Study of energy transfer processes between rare earth ions and photosensitizer molecules for photodynamic therapy with IR-excitation

D V Pominova,S V Kuznetsov,V B Loschenov,I D Romanishkin,T A Saveleva,A S Bogatova,V Y Proydakova,E V Akhlyustina,E A Lukyanets

doi:10.24931/2413-9432-2021-10-4-23-34

Abstract

Today, photodynamic therapy is one of the most promising minimally invasive methods of treatment of various diseases, including cancer. The main limitation of this method is the insufficient penetration into the tissue of laser radiation used to activate photosensitizer molecules, which makes it difficult to carry out therapy in the treatment of large or deep-seated tumors. In this regard, there is a great interest in the development of new strategies for photodynamic therapy using infrared radiation for excitation, the wavelengths of which fall into the “transparency window” of biological tissues. In this work, it was proposed to use upconversion NaGdF4 :Yb:Er nanoparticles (UCNP), which absorb infrared excitation and serve as a donor that transfers energy to the photosensitizer. Photosens and phthalosens were chosen as the most promising photosensitizers for the study. The aim of this work was to study the energy transfer processes between upconversion nanoparticles doped with rare-earth ions and photosensitizer molecules. in order to excite photosensitizers with IR radiation and carry out photodynamic therapy of deep-seated neoplasms. Using spectroscopic and time-resolved methods, it has been demonstrated that there is an efficient energy transfer between upconversion particles and photosensitizers phthalosens and photosens. The calculated efficiency of energy transfer by the Foerster mechanism was 41% for the UCNP + photosens system and 69% for the UCNP + phthalosens system. It has been experimentally and theoretically proved that there is a binding of photosensitizer molecules with UCNP by means of surfactants, leading to a reduction in the distance between them, due to which effective nonradiative energy transfer is realized. The generation of singlet oxygen by the phthalosens photosensitizer upon excitation by means of energy transfer from UCNP, excited at 980 nm wavelength of, has been demonstrated.

Highlights

Исследование процессов передачи энергии между редкоземельными ионами и молекулами фотосенсибилизаторов для задач фотодинамической терапии с возбуждением в ик-диапазоне which makes it difficult to carry out therapy in the treatment of large or deep-seated tumors
There is a great interest in the development of new strategies for photodynamic therapy using infrared radiation for excitation, the wavelengths of which fall into the “transparency window” of biological tissues
The aim of this work was to study the energy transfer processes between upconversion nanoparticles doped with rare-earth ions and photosensitizer molecules. in order to excite photosensitizers with IR radiation and carry out photodynamic therapy of deep-seated neoplasms

Summary

ОРИГИНАЛЬНЫЕ СТАТЬИ

Лощенов Исследование процессов передачи энергии между редкоземельными ионами и молекулами фотосенсибилизаторов для задач фотодинамической терапии с возбуждением в ик-диапазоне. Исследованы процессы передачи энергии между АКНЧ, легированными редкоземельными ионами, и молекулами ФС для верификации возможности возбуждения ФС ИК-излучением и проведения ФДТ глубокозалегающих новообразований. При помощи спектроскопических и время-разрешенных методов продемонстрировано, что наблюдается эффективная передача энергии между АКНЧ и ФС фталосенс и фотосенс. Экспериментально и теоретически доказано, что наблюдается связывание молекул ФС с АКНЧ посредством поверхностно-активного вещества, приводящее к сокращению расстояния между ними, за счет чего реализуется эффективная безызлучательная передача энергии. Для цитирования: Поминова Д.В., Богатова А.С., Пройдакова В.Ю., Романишкин И.Д., Ахлюстина Е.В., Кузнецов С.В., Савельева Т.А., Лукьянец Е.А., Лощенов В.Б. Исследование процессов передачи энергии между редкоземельными ионами и молекулами фотосенсибилизаторов для задач фотодинамической терапии с возбуждением в ИК-диапазоне // Biomedical Photonics.

Материалы и методы

Результаты и обсуждение

Образец Sample

Эффективность Фёрстеровской резонансной передачи энергии между