Abstract
Illicit use of narcotic drugs and psychotropic substances is becoming a major problem in India. People from different age groups are consuming illicit drugs and experiencing dependence and addiction problem. Cannabis is one such drug, a flowering plant having psychoactive nature due to presence of different cannabinoids. Its analysis in stepwise manner is essential for recognition. The forensic analysis is performed by using various techniques such as microscopy, chromatography, spectroscopy, etc. Currently, the forensic examination of cannabis is more focused on qualitative analysis in preliminary examination though quantitative analysis is also important which can play a major role in investigation. The present study focuses on analysis of cannabis sativa by color test, thin layer chromatography, UV visible spectroscopy and IR spectroscopy. Estimation of limit of detection for all methods for cannabis is the prime goal. According to the study, LoD for Fast Blue B and Duquenois Levine test is found to be 0.001 mg/mL and 0.125 mg/mL, respectively while LoD for thin layer chromatography and UV visible spectrophotometry is observed to be 0.5 mg/mL. and 0.10 mg/mL, respectively. The study helps to determine the sensitivity of all above mentioned techniques for cannabis examination along with its functional group identification.
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