Abstract
Background: The prevalence of disinfectant resistance in Pseudomonas aeruginosa is on the rise. P. aeruginosa is the most common bacteria isolated from cases of microbial keratitis. Many multi-purpose contact lens disinfectant solutions are available to decontaminate contact lenses before use and to help reduce the incidence of infections. However, with increasing disinfectant resistance, the effect of multi-purpose disinfectant solutions may diminish. The goal of this study was to examine genes associated with disinfectant resistance in ocular isolates of P. aeruginosa and understand the strain’s susceptibility to different multipurpose disinfectant solutions. Methods: Seven potential disinfectant resistance genes were used in BLASTn searches against the whole genomes of 13 eye isolates of P. aeruginosa. A microdilution broth method was used to examine susceptibility to four different multipurpose disinfectant solutions. Results: All strains possessed the sugE2, sugE3 and emrE (qacE) genes. The sugE1 and qacEdelta1 genes were present in 6/13 isolates. No strains contained the qacF or qacG genes. All tested disinfectant solutions had the ability to kill all test strains at 100% concentration, with some strains being susceptible at 1:8 dilutions of the disinfecting solutions. However, the presence of disinfectant resistance genes was not associated with susceptibility to multi-purpose disinfectants. Conclusion: All four tested contact lens disinfectant preparations are effective against P. aeruginosa isolates regardless of the presence of disinfectant resistance genes.
Highlights
Pseudomonas aeruginosa is a common isolate in eye infections [1]
P. aeruginosa is the major isolate from contact lens related infections and the antibiotic and disinfectant resistance in this species is on the rise [2]
We investigated the disinfectant resistance gene profile of ocular P. aeruginosa and susceptibility of these isolates to four Multi-purpose disinfectant solutions (MPDS) to find the association between the presence of disinfectant resistance genes and susceptibility to MPDS
Summary
Pseudomonas aeruginosa is a common isolate in eye infections [1]. A systematic review of P. aeruginosa in eye infections showed that about 20% of isolates in all eye infections are P. aeruginosa with the highest (68%) prevalence in contact lens associated keratitis (corneal ulcer) [2]. With wide array of pathogenic factors including exotoxins and proteases, P. aeruginosa keratitis is often fulminant and can rapidly destroy the cornea leading to vision loss [3]. This Gram-negative opportunistic bacterium utilises invasive and cytotoxic proteins to infect and damage corneal cells and cells of the immune system [4,5]. The goal of this study was to examine genes associated with disinfectant resistance in ocular isolates of P. aeruginosa and understand the strain’s susceptibility to different multipurpose disinfectant solutions. The sugE1 and qacEdelta genes were present in 6/13 isolates
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