Abstract
The present work was aimed to study the various factors affecting Agrobacterium tumefaciens mediated genetic transformation of three BARI masur variety of lentil (Lens culinaris Medik.), namely, BM-4, BM-5 and BM-6. Agrobacterium strain LBA4404 containing plasmid pBI121 which carrying gus and nptII as screenable and selectable marker genes, respectively, was used for transformation. Factors affecting transformation efficiency, such as, type of explants, bacterial density, incubation time and co-cultivation period were studied. The result showed that decapitated embryo (DE) explants of BM-6 variety of lentil inoculated in Agrobacterium suspensions at density of 1.0 (OD600) for 45 minutes and co-cultivated on MS medium with hormones in the dark for 3 days gave the highest transient expression of gus gene (86.90%). The lowest percentage (15.00%) of GUS +ve explants observed in cotyledonary node (CN) of BM-5 variety of lentil when they were inoculated in Agrobacterium suspensions at density of 0.5 (OD600) for 15 minutes. Using this protocol agronomical and economically important trait could be transfer to the locally grown lentil varieties.
Highlights
Lentils have been the part of the human diet since the aceramic Neolithic times
Three microsperma varieties of lentil (Lens culinaris Medik) namely, Bangladesh Agricultural Research Institute (BARI) Masur-4 (BM-4), BARI Masur-5 (BM-5) and BARI Masur-6 (BM-6) cultivated in Bangladesh were used as the plant materials for this investigation
Optimization of different parameters influencing transformation efficiency of explants Available reports on Agrobacterium-mediated genetic transformation indicate that, transformation efficiency is influenced by several factors such as optical density (O.D.) of Agrobacterium suspensions, incubation time, cocultivation period and culture media for co-cultivation of explants etc
Summary
Lentils have been the part of the human diet since the aceramic (before pottery) Neolithic times It is one of the first crops domesticated in the Near East and still the most important cool season annual pulse crops throughout the world. It is preferred over the other pulses by consumer’s preference all over the world. In Bangladesh, people like eating only red cotyledon type of lentil, where it is often eaten as a product “Dhal” as a main dish, side dish, salads or with flat bread (Roti) or rice Khichuri is another popular dish, which is made from a mixture of split lentil seeds and rice
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
