Study of danazol active agent
 effect on Mcf10a breast cells redox
 phosphorylation

Abstract

Relevance: Fibrocystic mastopathy is one of the
 most complex breast pathologies, occurring in about
 60% of women. The causes and molecular mechanisms of cyst formation in fibrocystic mastopathy are
 not fully understood, and effective treatment methods are not widely implemented. However, there is
 evidence of the fibrocystic mastopathy malignization
 risk. We studied the molecular effect of the active agent
 danazol on the respiratory complex in mitochondria,
 which occur in various concentrations and are considered the cell energy center.
 A method of permeation with special substances
 was developed to improve the cell membrane permeability in the intracellular proteins and nucleic acids
 analysis. The scientists aimed to study the Mcf10a cell
 line mitochondrial respiratory complex using the permeabilization method.
 Purpose: To study the effect of different danazol
 concentrations on Mcf10a breast cells redox phosphorylation in women with fibrocystic mastopathy.
 Results: Monitoring the redox phosphorylation
 process using the respiratory complex substrates
 affecting the Msf10A breast cells respiratory chain
 showed the danazol 30 μM effect on the respiratory
 complex I.
 Conclusions: According to oxygraphic records, 10
 minutes’ exposure to danazol 30 μM after permeabilization with digitonin increased the Msf10A cells’ oxidative phosphorylation compared with the control. The
 respiratory rate decreased by 45% ten minutes after
 treatment with digitonin.