STUDY OF CUCUMBER MOSAIC VIRUS GENE EXPRESSION IN CAPSICUM ANNUUM

Nur Hafizah Azizan,Ing Chia Phang,Zulkifli Ahmad Zainal Abidin

doi:10.26480/gws.02.2017.29.31

Abstract

Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli). Chilli plant samples exhibiting virus-like disease symptoms were collected from Taman Pertanian Indera Mahkota (location 1) and Greenhouse 12 of Horticulture Research Centre, Serdang (location 2). Viral disease was detected based on symptoms like mosaic-mottling, yellow ringspots and cholorotic that appeared on the leaves. The isolation of total RNA was done by using Vivantis GF-1 total RNA extraction kit. RT-PCR technique was used to detect the presence of virus disease symptoms gene in chillies. Identification of causal agents was based on cDNA amplified product size, using virus-specific oligonucleotides. Actin was used as the internal PCR control. The product size of the DNA fragment was 315 bp. From RT-PCR, the expression of CMV can be detected in chilli plants that exhibited the virus-like disease symptoms. This research revealed that some of the chilli plants at the Taman Pertanian Indera Mahkota and Greenhouse 12 of Horticulture Research Centre have been affected by this viral disease.

Highlights

Capsicum annum or chilli has been domesticated historically, for 7,000 years
Total RNA extracted with GF-1 Vivantis Total RNA Extraction Kit was intact, and no smearing was detected (Figure 1)
This showed that the ‘healthy chilli location 2 at lane 1 was identified as non-infected

Summary

Introduction

Capsicum annum or chilli has been domesticated historically, for 7,000 years. It has been considered as ancient crops [1]. In 1542, chilli was adopted by the local people, and it has been recorded as native to the India by a German botanist, Leonhard Fuchs. Capsicum annum, which originated from Mexico contains both, the large-fruited bell peppers and small pungent types, is the most widely cultivated species. It has become the predominant chilli globally, partly because it was the first chilli discovered by Columbus and introduced to the rest of the world. Chilli has been used to prepare local cuisine such as Malaysian or Indonesian sambal, Thai hot and sour soup, Korean kimchi and Indian curry [2]

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Conclusion