Study of combination of liquid organic waste on antifungal activity Bacillus sp. DB12

Abstract

This study examines the ability of liquid organic waste carriers to increase the antifungal activity of the active ingredient Bacillus sp DB12, which has been stored for 12 weeks against the pathogen Fusarium oxysporum f.sp. cepae. The research method was carried out in an experimental laboratory using a completely randomized design (CRD) with a single factor in the form of a liquid waste carrier consisting of 9 treatments. The composition of liquid tofu waste (TLW), coconut liquid waste (CLW), and rice washing liquid waste (RLW) was determined as follows: 100% TLW, 75% TLW + 25% CLW, 50% TLW + CLW 50%, TLW 25% + CLW 75%, CLW 100%, TLW 75% + RLW 25%, TLW 50% + RLW 50%, TLW 25% + 75% RLW, and 100% RLW. The results showed that the combination of 75% TLW + 25% CLW carriers increased the antifungal activity of Bacillus sp. DB12 compared to other carrier combinations. An increase in antifungal activity was indicated by an inhibition zone diameter of 13.03 mm during 12 weeks of storage.