Study of collagen synthesis function of repairing abdominal wall defects in rats by nano-bionic and anti-adhesive compound hernia patch

Abstract

Objective To evaluate the advantages and disadvantages of nano-bionic and anti-adhesive compound hernia patch to repair the collagen synthesis function of the abdominal wall hernia. Methods 90 adult male SD rats were randomly divided into 3 groups: NT group (nano-bionic and anti-adhesive compound hernia patch), PP group (polypropylene patch) and PE group (polyester patch), building a model of the abdominal wall hernia. The patch approximately 3 cm×2 cm in size was sutured to theabdominal wall defect. After the postoperative observation of rats, 10 rats were executed at 4, 6 and 8 weeks after surgery, respectively, and the formation of collagen tissue in the abdominal wall was observed. Shearing patch and its surrounding tissues for histologic observation and analysis of collagen protein expression, and Western bolt analysis of type I collagen and typeⅢ collagen expressionin abdominal wall repair area. Results The postoperative general conditions of rats were good, and there were no adverse events such as death. In the area of abdominal wall surgery, the NT group had soft touch and less foreign body sensation, and no obvious abdominal organ protruding, and the fibrous tissue was growingwell. The feeling of the PP group was more rigid, and there were a large number of subcutaneous neovascularization. The abdominal wall repair area of the PE group was stiff, and the growth of the fibrous tissue was better. Histological observation: at each time point, the repair effect of NT group was better than that of PP group and PE group. NT group had more type I collagen expression, and less typeⅢ collagen expression. It had more reasonableratio of typeⅠ/Ⅲ collagen, more mature and stable fiber structure. Tissue protein: TypeⅠ collagen gradually increased over time in each group; At each time points, the NT group was obviously higher than that of PP group and PE group; At 6 weeks and 8 weeks after surgery, the ratio of typeⅠ/Ⅲ collagen in the NT group was about 4∶1, significantly increasingcompared with 4 weeks after the operation. Conclusion In the process of routine tension-free repair of abdominal wall defect, the nano bionic, antiblocking compound hernia patch has earlier mature and stable fiber structurethan polypropylene patch and polyester patch, providing a more solid repair effect. It is applicable to the conventional abdominal wall hernia repair, especially in large abdominal wall defect caused by abdominal wall hernia. Key words: Abdominal wall hernia; Composite patch; Nano biomimetic material; Rat; Collagen