Study of caesalpinia sappan aqueous extract's apoptosis-inducing effects on human ovarian cancer cell SKOV3

Abstract

Objective To investigate the mechanism of oaesalpinia sappan aqueous extract's apoptosis-inducing on human ovarian cancer cell line SKOV3.Methods With different concentration of eaesalpinia sappan aqueous extract acting on cells,immunocytochemistry was used to detect the expression of Caspase-3,9 and survivin assay in ovarian cancer cell line SKOV3.Results The positive rate of Caspase-3,9 were increased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale was decreased.The positive rates of surviving were decreased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale were increased.At the concentration of 0,25 μg/ml,50μg,ml,100μs/ml,200μg/ml,the positive rates of Caspase-3 were 5.29%,12.61%,24.60%,100.00%,100.00%,respectively;the positive rates of Caspase-9 were 12.32%,29.17%,57.42%,100.00%,100.00%,respectively;the positive rates of survivin were 8 1.62%,60.53%,36.94%,55.69%,36.92%,respectively.Conclusion Caesalpinia sappan aqueous extract can obviously induce apoptosis of human ovarian cancer ceil line SKOV3 by increasing expression of Caspase-3,9 and decreasing expression of survivin. Key words: Ovarian neoplasms; Ceils; Apoptosis inducing factor; Lignum sappan