Abstract
Comparative therapeutic significance of tendon-derived stem cells (TDSCs) and bone marrow mesenchymal stem cells (BMSCs) transplantation to treat ruptured Achilles tendon was studied. Three groups of SD rats comprising 24 rats each, designated as TDSCs and BMSCs, and nontreated were studied for regenerative effects through morpho-histological evaluations and ultimate failure load. For possible mechanism in tendon repair/regeneration through TDSCs and BMSCs, we measured Collagen-I (Col-I), Col-III gene expression level by RT-PCR, and Tenascin-C expression via immunofluorescent assay. TDSCs showed higher agility in tendon healing with better appearance density and well-organized longitudinal fibrous structure, though BMSCs also showed positive effects. Initially the ultimate failure load was considerably higher in TDSCs than other two study groups during the weeks 1 and 2, but at week 4 it attained an average or healthy tendon strength of 30.2 N. Similar higher tendency in Col-I/III gene expression level during weeks 1, 2, and 4 was observed in TDSCs treated group with an upregulation of 1.5-fold and 1.1-fold than the other two study groups. Immunofluorescent assay revealed higher expression of Tenascin-C in TDSCs at week 1, while both TDSCs and BMSCs treated groups showed detectable CM-Dil-labelled cells at week 4. Compared with BMSCs, TDSCs showed higher regenerative potential while treating ruptured Achilles tendons in rats.
Highlights
About 30 million ligament and tendon injuries are reported annually across the globe due to lifestyle, recreation, work patterns, accidents, pharmacological agents, and degenerative biological variables such as gender, age, and genetics [1]
The results indicate that tendon-derived stem cells (TDSCs) exhibit a better-regenerative potential when compared with bone marrow mesenchymal stem cells (BMSCs) in treating ruptured Achilles tendons and could be a better alternative cell source for treating Achilles tendon
The differentiation analyses showed that the adipogenesis (Oil red), chondrogenesis (Toluidine blue), and osteogenesis (Alizarin red) of the isolated TDSCs and BMSCs were emerged after induction (Figure 2(b))
Summary
About 30 million ligament and tendon injuries are reported annually across the globe due to lifestyle, recreation, work patterns, accidents, pharmacological agents, and degenerative biological variables such as gender, age, and genetics [1]. Anatomical studies are crucial for in-depth understanding of tendon healing and regeneration. Tendon is comprised of parallel and well organized collagen (Col) bundles, of which approximately 90% are of Col-I, while the rest of the 10% are Col-III, -IV, -V, and -VI [2]. Chronic or acute tendon injuries are primarily treated with conservative or surgical treatments, where the former is used for symptomatic relief only, is ineffective and time-consuming, but later involves the use of autografts, allografts, xenografts, and prosthetic devices [3, 4]. It is crucial to define some innovative techniques to treat such tendon injuries
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
