Abstract

The influence of exogenous factors on the selectivity of biotransformation of cholesterol 3β-methyl ether by Mycobacterium sp. was studied. The main product of the microbiological transformation was Δ5-dehydroepiandrosterone 3β-methyl ether. The presence of methyl-β-cyclodextrin in the bacterial growth medium led to a change in the ratio of the reaction products and an increase in the yield of Δ4-androstene-3,17-dione. Aliphatic saturated and unsaturated carboxylic acids were also formed in the course of biotransformation.

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