Abstract
Frontal analysis is classically used to measure adsorption isotherms, and in this work the isotherms of Tröger's base were determined on microcrystalline cellulose triacetate with ethanol as solvent at 30, 40 and 50°C for the (−)-enantiomer and at 30°C for the (+)-enantiomer. The isotherms of the first eluted (−)-enantiomer are described satisfactorily by the Langimuir equation at all temperatures. In contrast, the isotherm of the longer retained (+)-enantiomer possesses a pronounced inflection point and can be described by a quadratic isotherm equation. However, besides supplying information about the adsorption equilibrium, frontal analysis data can be used to determine kinetic parameters. Using the equilibrium-dispersive model of chromatography, the apparent axial dispersion coefficient was derived by fitting the breakthrough curve for each concentration step. The values are in general agreement with those obtained from the classical determination of the efficiency of elution profiles under linear conditions. However, the apparent dispersion coefficient determined from frontal analysis depends on the concentration.
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