Abstract
Microorganisms known as Plant-associated Pseudomonas can thrive as parasites or saprophytes on plant surfaces and within plant tissues. They are crucial in enhancing plant growth by suppressing pathogenic microorganisms, synthesizing growth-stimulating plant hormones, and boosting plant disease resistance. At the University of Limpopo in South Africa, a molecular study was conducted in 2023 to identify a Pseudomonas bacterium in a spinach field associated with a free-living nematode, Acrobeles complexus, in Limpopo Province. The study utilized the Chelex method to extract DNA and the 16S rDNA marker to identify the bacterium as Pseudomonas fragi. Specific primers were then used to amplify 16S rDNA, which identified the bacterium, and Nblast analysis revealed a 96% similarity to a strain from India. Phylogenetic analysis placed this species with those identified as P. fragi in the same clade with highly supported (100) bootstrap values. This marker (16S rDNA) is recommended to use. But for a better understanding of P. fragi's phylogeny, the other DNA markers are recommended to identify this species.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callSimilar Papers
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
