Abstract
A retrospective study was conducted among out-patient and in-patient in Kathmandu medical collegeTeaching hospital of one month from the duration 2060-11-15 to 2060-12 -15. Mid-stream urine [MSU] of300 patient were cultured. Out of these, 75 patients’ urine were found to have significant bacterial growth.Total of five species of bacterial species isolated were viz.E.coli 37(33.3%), Proteus species 25(27.7%),Klebsiella species 15(16.6%), Staphylococcus aureus 8(8.8%) and Pseudomonas aureginosa 1(1.1%).Regarding their antibiotic sensitivity pattern, E. coli were sensitive to Nitrofurantoin 31 (83.8% ), Norfloxacin25 (67.5%), Ofloxacin 30(81.0%), Amoxycillin 16(43.2%), Nalidixic acid 15 (40.5%).proteus species weresensitive to Nitrofurantoin 15 (60.0%), Norfloxacin 15 (60.0%), Ofloxacin 12(48.0%), Amoxycillin 13(52.0%),Nalidixic acid 10 (40.0%), Ciprofloxacillin 12(48.0%). Klebsiella species were sensitive to Nitrofurantoin 9(60.0%), Norfloxacin 5 (33.3%), Oflaxin 6(40.0%), Amoxycillin 5(33.3%), Nalidixic acid 4 (26.6%),ciprofloxacillin 6(40.0%).Staphylloccus aureus were sensitive to Nitrofurantoin 8(100.0%), Norfloxacin 6(75.0%), Oflaxin 8(100.0%),Amoxycillin 7(87.5%), Nalidixic acid 6(75.0%), Ciprofloxacillin 8(100 %). And Pseudomonas aureginosawere resistant to all antibiotics which we are using in this research work.Key Words: Urine sample, Identification, Sensitivity pattern.
Highlights
Urine is a sterile ultrafiltrate of blood
In absence of urinary tract infection (UTI), it emerges from kidney and bladder free from microbes
During passage through distal urethra, a small number of bacteria may enter the urine as contaminants
Summary
Urine is a sterile ultrafiltrate of blood. In absence of urinary tract infection (UTI), it emerges from kidney and bladder free from microbes. During passage through distal urethra, a small number of bacteria may enter the urine as contaminants This is because normally renal tissues, bladder and proximal urethra are sterile but several species of bacteria may be found in distal urethra as transient micro flora, most of which are derived from faecal flora. UTI is among the most common bacterial infections that lead patients to seek medical care. Most of the people are illiterate & do not know antibiotics and its mechanism against bacteria and resistant developing mechanisms. They are not aware of effect of irrational use of drugs, wrong dose and dose taken insufficient length of time. The resistance antimicrobial agents have been still on treat infectious disease caused by resistant pathogens of generic origin and transferable bacterial species and genera
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