Study of B10 cells in the pathogenesis of the interaction between chronic obstructive pulmonary disease and periodontal disease

Abstract

Objective: To determine the ratio of ingerleukin(IL)-10 secreting regulatory B cells (B10 cells) and the levels of IL-10 in peripheral blood of periodontitis patients with or without chronic obstructive pulmonary disease (COPD), and to explore the function of B10 cells in the pathogenesis of the interaction between COPD and periodontitis. Methods: Forty-five periodontitis patients and healthy people were recruited and divided into three groups: COPD group, COPD+periodontitis group and health controls with 15 people in each group. Peripheral blood samples were collected to determine the B10 population. Another group of 93 periodontitis patients and healthy people were also collected and divided into the same 3 groups with 31 people in each group. Peripheral blood samples were collected to determine the cytokine IL-10. Results: After stimulation with lipopolysaccharide (LPS)+phorbol-12-myristate13-acetate+ionomycin+brefeldin A (PIB) for 5 h, the ratio of B10 in COPD+periodontitis group [(0.44±0.11)%] was significantly lower than that in the control group [(0.63±0.14)%] and periodontitis group [(0.62±0.13)%] (P<0.01), respectively. The ratio of B10 cells showed no significant difference between periodontitis group and healthy control group (P>0.05). After stimulation with LPS+PIB for 48 h, the ratio of B10 cells in COPD+periodontitis group [(7.59±1.33)%] was significantly lower than that in the periodontitis group [(10.14±2.02)%] and the healthy controls [(11.80±1.71)%] (P<0.01), respectively. The ratio of B10 cells in periodontitis group was significantly lower than that in the healthy controls (P<0.05). The levels of IL-10 in periodontitis group [(2.55±0.61) ng/L] and COPD+periodontitis group [(1.95±0.45) ng/L] were significantly lower than that in the control group [(3.96±1.15) ng/L] (P<0.01) respectively. Moreover, the level of IL-10 in COPD+periodontitis group was significantly lower than that in the periodontitis group (P<0.01). Conclusions: The dysfunction of immune system caused by the decreased proportion of B10 cells might be involved in the pathological process of COPD promoted by periodontitis.