Abstract
Mine water samples from North Bohemian Brown Coal Basin (NBBCB), the Czech Republic, were tested. These mine waters were taken from the drilled hydrological wells in the mentioned NBBCB area. The purpose of the analysis was to find an available method to evaluate the concentration of 16 polycyclic aromatic hydrocarbons (PAH) using available equipment. Mine water samples were taken in February and April 2020 from the NBBCB area. Samples were first concentrated using Solid Phase Extraction (SPE). The extracts were then analyzed by high performance liquid chromatography (HPLC) in conjunction with the diode array detector (DAD) external standard method. The standard ISO 17993 was used in the analysis procedure, which was optimized for the HPLC/DAD system. The original detection limit of 0.005 µg/l set by the standard was adjusted to 0.0025 µg/l for 16 PAHs by optimizing the method. The total content of 16 PAHs ranged from values below the detection limit to 0.7315 µg/l. All concentrations were below the limit values for groundwater. The results showed that the mine waters from the NBBCB area are not contaminated with PAHs. Keywords: Concentration of PAH; Diode array detector (DAD); High performance liquid chromatography (HPLC); Mine water; North Bohemian Brown Coal Basin (NBBCB). DOI 10.35180/gse-2020-0043
Highlights
The North Bohemian Brown Coal Basin (NBBCB) is the largest sub-Ore Mountains basin and is located between the Ore Mountains, the Doupov Mountains and the Bohemian Central Mountains, the Czech Republic
We chose a spectrophotometric diode array detector (DAD) detector for analysis, which allows for the analysis of all 16 polycyclic aromatic hydrocarbons (PAH) according to the United States Environmental Protection Agency (US EPA)
For the needs of analysis of mine water samples, we had to optimize the procedures for solid phase extraction (SPE) extraction of 16 PAHs and for the analytical method to determine the 16 PAHs using high performance liquid chromatography (HPLC)/DAD system, including the determination of suitable DAD detector wavelengths at which the highest sensitivity for 16 PAHs is achieved
Summary
The North Bohemian Brown Coal Basin (NBBCB) is the largest sub-Ore Mountains basin and is located between the Ore Mountains, the Doupov Mountains and the Bohemian Central Mountains, the Czech Republic. The source of mine water pollution is mainly their flow through the rock environment These waters contain increased amounts of SO42-, Fe2+, Fe3+ ions and have an acidic pH – mainly due to the oxidation of iron sulphides (pyrite, marcasite) and the transition of their soluble components into solution. Waters that have been in contact with coal have, in addition to excessive amounts of iron, sulfur and other ions, excessive amounts of dissolved and undissolved substances, such as coal dust and clay particles [2]. These waters can be GeoScience Engineering http://gse.vsb.cz
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