Abstract

The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition. However, transferring and acclimatizing the plantlets to under soil or ex vitro condition were difficult. This study investigated plant survival with anatomical changes in plantlets while transferring from in vitro to ex vitro conditions to investigate vascular cylinder variations. The ex vitro rooting of the in vitro regenerated shoots, after having been treated with 500 mg/L IBA, showed a success rate of 80 per cent. These plantlets were rooted and acclimatized simultaneously in ex vitro condition.

Highlights

  • The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition

  • (0.5-2.0 mg/L) and α-Naphthalene acetic acid were dipped in 3 forms of auxin solution that (NAA) (0.2-0.5 mg/L) or only indole-3-butyric were prepared in different concentration from acid (IBA) (0.5-2.0 mg/L) for shoot induction. 100 to 700 mg/L for 3 mins

  • It was reported that the combination of BAP and NAA was useful for regeneration of Sophora toromiro [5]

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Summary

Introduction

The success of shoot and rooting from the nodal shoot segments of Sophora alopecuroides L. were induced in vitro condition. Collection of plant material and Effect of auxins on rooting of proliferated preparation of explants shoots (in vitro) (0.5-2.0 mg/L) and α-Naphthalene acetic acid were dipped in 3 forms of auxin solution that (NAA) (0.2-0.5 mg/L) or only indole-3-butyric were prepared in different concentration from acid (IBA) (0.5-2.0 mg/L) for shoot induction.

Results
Conclusion
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