STUDY OF AMINO ACID COMPOSITION IN ABOVE-GROUND PART INULA OCULUS CHRISTI L.

Abstract

One of the conditions for the introduction of a new type of medicinal plant raw materials into medical practice is a comprehensive study of its chemical composition. The wide distribution of amino acids in plants and their high biological activity contribute to the effective action of medicinal raw materials and preparations derived from it on the body. Amino acids give other biologically active substances an easily digestible form, while simultaneously potentiating their pharmacological effect. The world around us contains many amino acids, but about 20 are enough for people to function normally. Amino acids as constituents of proteins are involved in all life processes along with nucleic acids, carbohydrates and lipids. In addition to amino acids that make up proteins, living organisms have a constant reserve of free amino acids contained in tissues and cell sap. They are in dynamic equilibrium with numerous exchange reactions. Amino acids are used in the biosynthesis of phosphatides, porphins, nucleotides. The qualitative and quantitative composition of amino acids in the aerial part of Inula oculus christi was studied. The analysis was carried out on an AAA400 amino acid analyzer, a highly specialized automated liquid chromatograph with computer control, equipped with a post-column detector system. 0.2 g of a sample (accurately weighed on an analytical balance) was suspended in a 20 ml penicillin vial, added to the top with normal hydrochloric acid, tightly closed with a rubber lid, and placed in an oven for 23 hours at 110°C. After hydrolysis, it was cooled to room temperature and evaporated to dryness in a rotary evaporator, after which 5 ml of purified water was added and evaporated again. This water wash procedure is necessary to get rid of residual hydrochloric acid, which negatively affects yield and peak separation. To the sample evaporated to dryness, 50 ml of loading buffer (pH 2.2) was added. Before injection into the ion exchange column, the sample was filtered through a 0.2 µm syringe filter or blue ribbon filter paper. As a result of the analysis, 16 amino acids were found, of which 7 a.re essenti.al (valine, isoleucine, leucine, lysine, methionine, threonine, phenylalanine) and 9 nonessential (alanine, arginine, aspartic acid, histidine, glycine, glutamic acid, proline, serine, tyrosine).