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Abstract
Purification of the alkaline phosphatase from serum for people living with polycystic ovaries were used gel filtration technique using the gel Sephadex column G100 with dimensions (20 × 2) cm, for the purpose of purification of alkaline phosphatase the use of a buffer of the (Tris -HCl) with a pH (7.2) to separate، enzyme kinetics, where studied the effect of different concentrations of substrate (sodium bi-article basis Phenyl phosphatases), results showed increasing the activity of alkaline phosphatases when increased substrate concentration. The resulting shape is hyperbolic. Km (1.14) mM, also studied the effect of pH and found that the optimal pH of the enzyme is (10). The optimum temperature was 37 ° C and the effect of reaction time was 20 minutes. The effect of Ascorbic acid show it works as an Inhibitor to the purified enzyme as that type of inhibition is non-competitive inhibitor type.
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