Study of acute and subacute toxicity of an immunostimulating drug based on the S-derivative of 1,2,4-triazole

Abstract

In the course of studies of the acute (subacute) toxicity of the newly created drug “Immunokor”, based on the thioderivative 1,2,4-triazole, it was found that with a single oral and intramuscular administration, the DL50 was over 5000 mg/kg b.w. According to the currently existing classification, the drug is classified as toxicity class IV or “low-toxic substances.” During clinical examination of animals, against the background of the action of the studied agent, it was found that the state of the central nervous system and other functional organs in the body of laboratory rats was preserved. Reflex and motor activity had a character appropriate for this type of animal. Significant fluctuations marked body temperature, heart rate (pulse), and respiratory movements but generally were within the reference values. The coats of white rats of the control and experimental groups had preserved color and shine. When applying the studied immunotropic agent to the skin and mucous membranes, there were no local or post-resorptive reactions. When studying the effect of the drug “Immunokor” on the body of laboratory animals, it was found that 28 daily intramuscular administration of the drug did not cause either the death of the experimental animals or the manifestation of clinical signs of the disease. At the same time, it was found that in animals of the 2nd and 3rd experimental groups (when administered 5 and 10 times the therapeutic doses of the studied agent), the weight coefficients of the spleen, heart, and kidneys slightly increased, and the liver mass decreased. The latter, especially in group D3, had a dark red color with spot and focal character inclusions upon macroscopic examination. In addition, it was found that the use of the drug “Immunokor” in a 10-fold therapeutic dose caused an increase in the number of platelets, the number of lymphocytes and monocytes in the blood, and the blood serum - the level of urea, creatinine, and the activity of AST and ALT, against the background of a tendency to decrease in the concentration of hemoglobin, the number of erythrocytes and leukocytes, the value of hematocrit, the average concentration of hemoglobin in the erythrocyte (MCHC), the average content of hemoglobin in the erythrocyte (MCH), the average volume of the erythrocyte (MCV), the number of granulocytes and the concentration of total protein. The identified individual minor changes in hematological and individual biochemical indicators of the blood, as well as changes in the weight coefficients of the liver, kidneys, spleen, and heart, were of a short-term and restorative nature and were, apparently, the result of the body's compensatory reaction to the intake of a significant volume and concentration of the studied substance into the body of laboratory animals.