Abstract
Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H2O2 were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC) test, chick chorioallantoic membrane (CAM) assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch tests. The optimum extract was obtained from bean sprouts grown to 0.5 cm. Extracts of black bean sprouts are safe and can be used as additives in anti-aging and whitening cosmetic products.
Highlights
Black soybean (Glycine max var) is the black seed of the soybean Glycine max (L.) merr, known as the black bean
No signs of allergic reactions were observed in the human patch tests
The components which play a major role in the antioxidant and skin-whitening effects of black soybean remain to be elucidated
Summary
Black soybean (Glycine max var) is the black seed of the soybean Glycine max (L.) merr, known as the black bean. Studies have shown that more protein can be extracted from the bud of germinated black beans. Black bean sprouts are rich in calcium, phosphorus, iron, potassium and vitamins, where the level of vitamins increases in the budding process [3]. This makes the extraction easier: germination is a natural way to extract the active ingredient in the seeds, and the ingredients are easy absorbed by the body [4]. Most studies of black bean sprouts have mainly concentrated on their material composition and development as a functional food. Little research has been devoted to the use of black bean sprouts in beauty products
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