Abstract
The performance of a tyrosinase-modified “solid carbon paste” electrode (SCPE) as electrochemical detector has been studied in comparison with a glassy carbon electrode detector in high-performance liquid chromatography for the simultaneous determination of dopamine, 3,4-dihydroxyphenylacetic acid, norepinephrine and homovanillic acid. The influence of pH, flow-rate and amount of organic solvent in the mobile phase on the biosensor response was investigated. The stability and selectivity of the detector were significantly affected by the mobile phase pH. No effect of 2% isopropanol in the mobile phase was observed. The biosensor response was fast, reproducible, highly sensitive and linear over the concentration range 0.09 μM−1 m M (detection limit of ca. 290 pg of neurotransmitter injected).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
