Abstract
Conventional approaches to human intracellular optical sensing, generally, require dedicated laboratories with bulky detection systems. They are performed by cell labeling procedures based on the use of fluorophores that are, mostly, phototoxic, invasive, bleached in case of prolonged light exposures, which require carriers and/or structural modifications for the cellular uptake. These issues, together with the sensitivity of the eukaryotic cell model, could be problematic towards the development of a robust sensing system suitable for biomedical screening. In this work, we studied a sensing system resulting from the combination of the commercial tris(2,2’bipyridyl)ruthenium(II) fluorophore, for cell labeling, with a potentially miniaturizable optical system composed by a laser source and a photomultiplier tube, for the fluorescence analysis.
Highlights
Optical sensing applied to the human intracellular environment represents the best way for the analysis of cell functioning and its behavior in response to specific physiological conditions.Cell ultrastructure, embryogenic development [1], pH–calcium–chloride–oxygen monitoring [2] and, most importantly, metastasis proliferation [3] are a few examples of what this type of optical sensors would help to analyze.Intracellular optical sensing works by the introduction of a luminescent probe inside the target cell and its subsequent detection.The configuration of an optical sensing strategy, generally, starts with the fluorophore choice and its characterization, mainly consisting ofAppl
The magnification of microscopy revealed the intracellular distribution of fluorophore, confirming blue light (488 nm), while cells treated with the fluorophore (Figure 8B) showed the Ru(bpy)32+
The magnification of microscopy revealed the intracellular distribution of fluorophore, confirming the success of the uptake procedure and its reproducibility in a different cell model
Summary
Optical sensing applied to the human intracellular environment represents the best way for the analysis of cell functioning and its behavior in response to specific physiological conditions.Cell ultrastructure, embryogenic development [1], pH–calcium–chloride–oxygen monitoring [2] and, most importantly, metastasis proliferation [3] are a few examples of what this type of optical sensors would help to analyze.Intracellular optical sensing works by the introduction of a luminescent (fluorescent or chemiluminescent) probe inside the target cell and its subsequent detection.The configuration of an optical sensing strategy, generally, starts with the fluorophore choice (considering its optical and biochemical properties) and its characterization, mainly consisting ofAppl. Optical sensing applied to the human intracellular environment represents the best way for the analysis of cell functioning and its behavior in response to specific physiological conditions. Embryogenic development [1], pH–calcium–chloride–oxygen monitoring [2] and, most importantly, metastasis proliferation [3] are a few examples of what this type of optical sensors would help to analyze. Intracellular optical sensing works by the introduction of a luminescent (fluorescent or chemiluminescent) probe inside the target cell and its subsequent detection. The configuration of an optical sensing strategy, generally, starts with the fluorophore choice (considering its optical and biochemical properties) and its characterization, mainly consisting of.
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