Study of a microwave digestion method for total arsenic determination in marine mussels by electrothermal atomic absorption spectrometry: application to samples from the Ria de Arousa

Abstract

Arsenic determination in mussel tissue was performed by electrothermal atomic absorption spectrometry (ETAAS) with Zeeman background correction and using iridium as a chemical modifier. Samples were digested by microwave heating using a mixture of nitric and sulphuric acids. This mixture makes possible the destruction of organoarsenic compounds, specifically arsenobetaine, prior to the graphite furnace determination. Optimum pyrolysis and atomization temperatures were 1,100 and 1,800 °C, respectively. The method was precise (with RSD% < 10), accurate (study of a certified reference material: 18.4 ± 1.4 μg As g−1 vs. a certified content: 18.0 ± 1.1 μg As g−1; recoveries between 90 and 104%) and sensitive (LOD 0.21 μg g−1 on a dry weight basis). The method was applied to the determination of arsenic in aquaculture mussels collected in four sampling campaigns from the productive Ria de Arousa (estuary sited in Galicia, NW of Spain).