Abstract
The generation of an engineering strain with reduced genome relies on efficient methods for marker-free gene knockout. In this study, research on the efficiency of deletion of genome fragments with upp-based counterselective method in Bacillus subtilis was carried out. Using this method, the 3.053 Kb, 12.414 Kb and 34.148 Kb fragments of the skin element were marker-free deleted successfully, with the efficiency of 27%, 11.4% and 25%, respectively. Here we demonstrate that this method is an efficient genetic tool for large genome fragments marker-free deletion.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
