Abstract

A strain of green streptococcus, No. 403, obtained from a case of bovine mastitis and similar to that usually associated with chronic mastitis in cattle, has under stated conditions been passed through Berkefeld N and Chamberland L3, L5 filters. It has been recovered from the filtrates and proved to be identical in its biological characteristics with the original unfiltered strain No. 403.Filtrates of this organism grown in K medium have given the largest number of recoveries, these being 18 out of 20, or 90%. Filtrates of growth in nutrient broth were recovered in 1 out of 20 filtrations, or 5%.Observations on the cultural and morphological appearance of the organism during the stages of its recovery are made. The interpretation of these experiments in the light of Zinsser's postulates is discussed.

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