Abstract
Based on the phenomenon of chlorophyll a photoluminescence, this paper introduces a new method to measure the chlorophyll a content, using 660nm laser diode as a new kind of light source to stimulate fluorescence as well as combining a fiber and spectrum technique. We analyze the characteristics of laser-induced fluorescence spectrum of chlorophyll a and then put forward the new method using two parameters, the relative fluorescence intensity and fluorescence intensity ratio F685/F735, to measure the chlorophyll a content in the water and green leaves respectively. The experimental results indicate that it is completely feasible to give a visual judgment for chlorophyll a content, according to the fluorescence emission spectrum of chlorophyll a. Subsequently, it is verified by three kinds of typical applications. All of these provide a new kind of light source to develop the chlorophyll a fluorometry and further give a technical foundation of on-spot monitoring the chlorophyll a content in the ocean or in green leaves.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
