Study in inactivation of porcine parvovirus in human coagulation factor VIII by short-wave ultraviolet-C

Abstract

Objective To investigate the effect of short-wave ultraviolet-C (UV-C) on inactivating porcine parvovirus (PPV) in human coagulation factor Ⅷ (FⅧ). Methods PPV was mixed with the FⅧ intermediate as an indicator virus. The FⅧ intermediates containing PPVs were inactivated using UVivatec instrument with 200, 300, and 400 J/m2 UV-C. The FⅧs inactivated by UV-C were inoculated in ST cells and cultured. PPV was detected by cytopathic effect (CPE) method and the virus titer was calculated by Reed-Muench method. The samples without CPE were cultured 3 blind passages to verify the inactivation effect. At the same time, the FⅧ activities before and after UV-C inactivation were detected. Results The PPV titers in FⅧ all decreased no less than 4.62 lg 50% tissue culture infective dose per0.1 ml after UV-C inactivation with 3 exposure doses. PPVs were not detected in the samples without CPE cultured to 3rd blind passage. The activities of FⅧ inactivated by UV-C did not decrease significantly, and each quality index met the requirements of pharmacopeia. Conclusion UV-C can inactivate nonenveloped virus PPV, and has no influence on the FⅧ activity. Key words: Ultraviolet rays; Virus inactivation; Parvovirus, porcine; Factor Ⅷ