Abstract
Cytochrome c oxidase subunit II, also known as cytochrome c oxidase polypeptide II which is an oligomeric enzyme. In this study Cytochrome c oxidase subunit 2 (mitochondrion) protein has been used to investigate its role in antigenicity. Cytochrome c oxidase subunit 2 protein sequences (230 aa protein) is analyzed through different types B- cell epitope prediction methods. We found that the region of maximal hydrophilicity is likely to be an antigenic site, having hydrophobic characteristics, because the terminal regions of antigen protein is solvent accessible and unstructured, antibodies against those regions are also likely to recognize the native protein. It was seen that an antigen protein is hydrophobic in nature and contains segments of low complexity and high-predicted flexibility. The predicted antigenic protein segments of Cytochrome c oxidase subunit 2 can take active part in the host immune reactions. In future study the predicted antigenic protein Cytochrome c oxidase subunit 2 fragments can be used in the investigation of MHC molecules binding and it can be the first bottlenecks in vaccine design.
Highlights
Dracunculiasis, is caused by a 60-100 cm long nematode worm, Dracunculus medinensis, via a drinking of contaminated water infected with copepod Cyclops
The HoppWoods scale Hydrophilicity Prediction Result Data found high in position between 55-60 (55-SFEDDY-60) in a protein, assuming that the antigenic determinants would be exposed on the surface of the protein and would be located in hydrophilic regions (Figure 2)
We study Hydrophobicity plot of HPLC / Parker Hydrophilicity Prediction Result Data found between 111-115 (Maximum Score 5.1) i.e., the maximum predicted residues at the position 115(Residue is D) is 112- EYSDYTD-118 and at position 116(Residue is Y) with start and end position is 113YSDYTDE-119 (Figure 4), BepiPred predicts the location of linear
Summary
Dracunculiasis, is caused by a 60-100 cm long nematode worm, Dracunculus medinensis, via a drinking of contaminated water infected with copepod Cyclops (intermediate host). Guinea worm the largest tissue parasite with unusual life cycle with incubation period of the approximately more than a year with six developmental stages. This one of the most neglected tropic parasite which bears clinical importance and needs to be eradicated after small pox [1]. After an incubation period the female worm release the larvae which induces a painful blister (1 to 6 cm diameter) on the skin of lower limbs; the person develop a slight fever, local skin redness, swelling and severe pruritus around the blister. Immersing or pouring water over the blister provides pain relief This the moment that adult female is exposed to the external environment [4]. During emergence of the limbs in open water sources it recognizes the temperature difference and releases the milky white liquid in the water which contains millions of immature larvae, when larvae released in water are ingested by copepods where they mount twice and become infective larvae within two weeks [5]
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