Abstract

Rapid and reliable temperature change is required for the study of temperature-dependent channel gating mechanisms. We use continuous near-infrared (CNI) laser as a heat source to rapidly increase the local temperature at a cell or a cell-free patch membrane containing temperature-sensitive TRP channels. The photothermal effects of laser irradiation can be characterized by transient changes in fluorophore emission, liquid junction potential, and function of membrane proteins. The CNI laser-based method is advantageous over perfusion-based heating methods mainly in speed but also in its reproducible temperature raising profile and minimal interference with perfused solutions containing channel agonists/antagonists.View Large Image | View Hi-Res Image | Download PowerPoint Slide

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