Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells

Hewida Hassan Fadel

doi:10.11648/j.crj.20180603.14

Abstract

In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (P<0.001) as well as ES (P<0.05). The serum level of CECs decreased significantly (P<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.

Highlights

Breast cancer (BC) is the greatest lethal cancer among women worldwide
It is noticed that there is a decrease of adverse effect of chemotherapy as vomiting, fatigue and nausea in Gp-IIb comparing to Gp-IIa
The present study reveals that the anti-angiogenic action of vitamin D3 and B6 isn’t limited to reduce angiogenesis via decreasing Vascular Endothelial Growth Factor (VEGF) level and keep ES within its active level suggesting that the combination of vitamins D3 and B6 strengthen the anti-angiogenic action of chemotherapy and interfere with metastasis in BC patients

Summary

Introduction

Breast cancer (BC) is the greatest lethal cancer among women worldwide. In Egypt, BC incidence is estimated for37.7% of all reported tumor and high mortality rate in addition to the aggressiveness of disease due to limited access of early diagnosis in rural areas [1]. Several risk factors increase the incidence of BC as age, reproductive and menstrual history, hormonal factors, family history, lifestyle, 93 Hewida Hassan Fadel et al.: Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells environmental factors and race [2]. Continuous generation of ROS within mammalian cells enhances the tumor cell production of the angiogenic factors which lead to increase risk factor of cell motility, invasion, metastasis, recurrence and aggressiveness of BC. Tumor cells secrete a large amount of pro-angiogenic factors, of these; Vascular Endothelial Growth Factor (VEGF) which plays the major role. Losing of angiogenic balance in cancer cells directed several studies to focus on inhibition of angiogenesis that is the most important factor in the progression of cancer as well as the most promising novel approaches for the treatment of cancer [5]

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