Study about the distinct expression of serum proteins between Uygur renal cell cancer and Han renal cell cancer

Abstract

Objective To observe serum protein change between Uygur with renal cell cancer (RCC) and Han with RCC and to construct different ethnic(including Han and Uygur) diagnostic model of RCC in XinJiang and screen serum differentially expressed protein between Han with renal cancer and Uygur with renal cancer in Xinjiang;Methods To use weak cation exchange and hydrophobic surface protein chip (CM10) and Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) to detect the serum protein.To detected serum specimens from patients (36 Han and 24 Uygur)with RCC and 60 healthy controls so as to construct different ethnic RCC diagnostic model and screen out disparate serum proteins between Han with RCC and Uygur with RCC.Results Obvious disparity existed between healthy controls and RCC patients in Uygur.Diagnostic model was composed and established by six proteins (5935,5945,5911,13 766,13 965,6887).The specificity and sensitivity were 91.67％ (22/24) and 100.00％ (24/24) ; Obvious disparity existed between healthy controls and RCC patients in Han.Total were four proteins peaks (5937,5345,5947,5912),and diagnostic model composed and established.The specificity and sensitivity were 94.44％ (34/36)and 91.67％ (33/36) ;disparity existed between Uygur RCC patients and Han RCC patients in terms of serum protein finger prints.Three proteins peaks M/Z 6887,13 766,13 965 existed diagnostic model of Uygur patients group while lacked in Han patients group,meanwhile,M/Z 5345 existed diagnostic model of Han patients group while lacked in Uygur patients group.Conclusion It has difference for protein finger prints between Han with RCC and Uygur with RCC in Xinjing.M/Z 6887,13 766,13 965,5345 may be differential markers between Uygur with RCC and Han with RCC and M/Z 6887,13 766,13 965 may be Keratin,transthyretin and interferon-inducedtransmembrane protein-1. Key words: Renal cell carcinoma; Han; Uygur; Proteomics