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Abstract
A TECHNIQUE has been developed in this laboratory for the rapid labelling of erythrocytes in vivo and in vitro using p-iodophenylhydroxylamine (I.C6H4.NH.OH) containing radioactive iodine. This compound, together with its green oxidation product p-iodonitrosobenzene (I.C6H4.NO), has a remarkable affinity for haemoglobin, and in spite of its insolubility in water rapidly penetrates red cells and combines firmly and stoichiometrically with the cell pigment1. The reaction with haemoglobin and some of its derivatives has been examined in detail and appears to be irreversible so long as the haem molecule remains intact.
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