Studies on uroparotin. IV. Isolation and some properties of uroparotin.

Abstract

By means of isoelectric precipitation, ethanol fractionation, and alumina treatment method, uroparotin was isolated in a homogeneous form from human urine. The yield of uroparotin was shown to be 1-1.2mg per liter for α-uroparotin and 0.8mg per liter β-uroparotin. Uroparotin was considered to be a typical glycoprotein, containing sialic acid as one component of carbohydrate moieties. Neither a proteolytic effect nor a blood pressure depressing effect was observed in uroparotin, nor other hormonal actions. Therefore, uroparotin may be considered a new glycoprotein having characteristic biological effects. No difference in properties of both α-and β-uroparotin could be found, and the mechanism of the biological effects remains to be clarified.