Studies on Trypsin and Chymotrypsin Inhibitory Activities, Hemagglutinating Activity, and Sugars in the Great Northern Beans (Phaseolus vulgaris L)

Abstract

ABSTRACTTrypsin and chymotrypsin inhibitory activities, hemagglutinating activity, oligosaccharides, total and reducing sugars were determined in Great Northern beans (Phaseolus vulgaris L.) flour, albumins, globulins, protein concentrates, and protein isolates. The bean flour and the protein concentrates were further evaluated for hydrogen gas prod action following ingestion in rats. The trypsin and chymotrypsin inhibitory activities were reduced in protein concentrates (75.32 and 84.78% reduction, respectively), and protein isolates (97.98 and 85.39% reduction, respectively), Albumins, globulins, protein concentrates, and protein isolates did not contain raffinose and vertascose while stachyose was present (14.69 mg/g) only in the albumins. Protein concentrates were essentially void of flatulence activity. Hemagglutinating activity was absent in albumins, globulins, protein concentrates, and protein isolates, but was present in the beaii flour.