Abstract
BackgroundThe present study describes the tracheorelaxant and anti-inflammatory effects of Polygonatum verticillatum which may support its medicinal use in hyperactive airway complaints and inflammatory disorders.MethodsThe tracheorelaxant activity of crude extract of the rhizomes of P. verticillatum (PR) was assessed in isolated guinea-pig tracheal tissues immersed in tissue organ bath filled with Tyrode’s solution and a continuous supply of carbogen gas (95% O2 and 5% CO2). The contractile and relaxant responses of the tissue were measured using isometric transducers coupled with Power-Lab data acquisition system. The anti-inflammatory effect was evaluated in carrageenan-induced rat paw edema model, while the lipoxygenase inhibitory activity was performed in the in-vitro assay. Various chromatographic and spectroscopic techniques were used for the isolation and characterization of pure molecules.ResultsIn isolated guinea-pig tracheal preparations, PR caused complete inhibition of the high K+ (80 mM) and carbachol-induced contractions however, it was more potent against K+ than CCh, similar to verapamil. Pretreatment of the tissue with PR, displaced the Ca2+ concentration-response curves to the right, similar to that induced by verapamil, indicating the presence of Ca2+ channel blocking like activity. When tested on carrageenan-induced rat paw edema, PR demonstrated a marked reduction in edema with 65.22% protection at 200 mg/kg, similar to aspirin. In the in-vitro assay, PR showed lipoxygenase inhibitory activity (IC50: 102 ± 0.19 μg/mL), similar to baicalein. Bioactivity-guided fractionation led to the isolation of 2-hydroxybenzoic acid and β-sitosterol.ConclusionsThese results indicate that the plant possesses tracheorelaxant, mediated possibly through a Ca2+ channel blockade mechanism, and anti-inflammatory activities, which may explain the medicinal use of this plant in airway disorders and inflammation.
Highlights
The present study describes the tracheorelaxant and anti-inflammatory effects of Polygonatum verticillatum which may support its medicinal use in hyperactive airway complaints and inflammatory disorders
This study describes the tracheorelaxant and antiinflammatory activities of P. verticillatum to provide a scientific background to its medicinal use in hyperactive airways complaints like asthma or inflammatory conditions
Pretreatment of P. verticillatum (PR) at the doses of 0.03 and 0.1 mg/mL, displaced the concentration-response curves (CRCs) of Ca2+ to the right with suppression of the maximum response (Figure 1b), similar to that caused by verapamil, which displaced the Ca2+ CRCs to the right with suppression of the maximum response at tested doses of 0.003 and 0.01 μM (Figure 1d)
Summary
The present study describes the tracheorelaxant and anti-inflammatory effects of Polygonatum verticillatum which may support its medicinal use in hyperactive airway complaints and inflammatory disorders. In different traditional systems of medicine, Polygonatum is popular for its use in pulmonary disorders like asthma and inflammation [3,4], in addition to its multiple other health benefits such as, antituberculant, antidiabetic, antihypertensive, diuretic,. A variety of phytochemical constituents have been isolated from different species of the genus Polygonatum; primarily: saponins, alkaloids, glycosides, flavonoids and phytohormones. These groups of compounds show different types of activities. This study describes the tracheorelaxant and antiinflammatory activities of P. verticillatum to provide a scientific background to its medicinal use in hyperactive airways complaints like asthma or inflammatory conditions. The in-vivo and in-vitro experimental studies have been designed, followed by bioactivity-guided isolation of its secondary metabolites
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