Abstract
The MTT test is used to determine the cytotoxicity of species: <em>Achlya americana, A. caroliniana, Leptomitus lacteus, Saprolegnia ferax</em> and <em>S. lapponica</em>, which occurred in Lake Sitno in the Drawieński National Park (W Poland). It was stated that among five examined species of zoosporic fungi only one of them <em>A. caroliniana</em>, does not show cytotoxic effect. Low and mean toxicity of other tested water FLO, however, combined with the large-scale character of their appearance shows, that they can create serious threat for the spawn, fry, fish as well as the other organisms (including invertebrates) existing in water.
Highlights
Water fungi play a crucial part in the biological decomposition of dead plant and animal remains – detritus, in water reservoirs
fungus-like organisms (FLO) cultures were used in research for toxicity, with application of tetrazole salt MTT (3[4,5-dimetyltiazol-2-yl]-2,5-difenyltetrazlium bromide, Sigma Aldrich), and with application of cells of kidneys obtained from swine (SK), after they were extracted with chloroform
Leptomitus lacteus showed to be of medium-toxicity (Tab. 1), with the IC 50 cytotoxicity level of 4.91 cm2/ml
Summary
Water fungi play a crucial part in the biological decomposition of dead plant and animal remains – detritus, in water reservoirs. Research on water fungi present on muscular tissues and on fish spawn is so far very fragmentary in Poland. Water fungus-like organisms (FLO) species common in reservoirs: Achlya americana Hamphrey, A. caroliniana Coker, L e p tomitus lacteus Agardh, Saprolegnia ferax (Gruith.) Thuret and S. lapponica Gäum.
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