Abstract

The MTT test is used to determine the cytotoxicity of species: <em>Achlya americana, A. caroliniana, Leptomitus lacteus, Saprolegnia ferax</em> and <em>S. lapponica</em>, which occurred in Lake Sitno in the Drawieński National Park (W Poland). It was stated that among five examined species of zoosporic fungi only one of them <em>A. caroliniana</em>, does not show cytotoxic effect. Low and mean toxicity of other tested water FLO, however, combined with the large-scale character of their appearance shows, that they can create serious threat for the spawn, fry, fish as well as the other organisms (including invertebrates) existing in water.

Highlights

  • Water fungi play a crucial part in the biological decomposition of dead plant and animal remains – detritus, in water reservoirs

  • fungus-like organisms (FLO) cultures were used in research for toxicity, with application of tetrazole salt MTT (3[4,5-dimetyltiazol-2-yl]-2,5-difenyltetrazlium bromide, Sigma Aldrich), and with application of cells of kidneys obtained from swine (SK), after they were extracted with chloroform

  • Leptomitus lacteus showed to be of medium-toxicity (Tab. 1), with the IC 50 cytotoxicity level of 4.91 cm2/ml

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Summary

Introduction

Water fungi play a crucial part in the biological decomposition of dead plant and animal remains – detritus, in water reservoirs. Research on water fungi present on muscular tissues and on fish spawn is so far very fragmentary in Poland. Water fungus-like organisms (FLO) species common in reservoirs: Achlya americana Hamphrey, A. caroliniana Coker, L e p tomitus lacteus Agardh, Saprolegnia ferax (Gruith.) Thuret and S. lapponica Gäum.

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