Abstract
ABSTRACT. The ability of synaptosomes prepared from rat brain tissue to accumulate pglu-his-[2,3-3H]pro-NH2 ([3H]TRH), a possible peptidergic neurotransmitter, was investigated. The uptake of [3H]- dopamine, a proven neurotransmitter, was evaluated in parallel experiments. When a synaptosome preparation derived from homogenates of male rat hypothalamic or cerebrocortical tissue was diluted with an equal volume of Hanks' balanced salt solution and incubated with [3H]TRH, uptake of a radiolabeled substance(s) by subcellular particles was demonstrated. Similar results were obtained in mixtures containing [3H]dopamine. The particles accumulating radiolabeled compounds were identified as synaptosomes on the basis of size, ultrastructural profiles, and sedimentation characteristics on continuous and discontinuous sucrose density gradients. The uptake of a radiolabeled compound(s) from incubation media containing [3H]TRH was dependent on temperature, duration of the incubation period, presence of glucose and ele...
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