Abstract
The specific activity of thymidine kinase was found to increase during the replication of polyoma virus in mouse embryo cells. The rise in enzyme activity was observed between 8 and 10 hours after infection (2 hours before the onset of viral DNA synthesis). Puromycin abolished the elevation of thymidine kinase activity, suggesting that it is due to de novo enzyme synthesis. Some of the properties of the thymidine kinase formed during polyoma virus replication were found to differ from those of the enzyme present in uninfected cells.
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