Studies on the Teratogenicity of Food Additives (3)

Abstract

The teratogenic effects of cyelohexylamine (CHA) and cyclohexylamine sulfate (CHA-sulfate) were examined in rats. The results obtained were as follows:Adult Wistar strain female and male rats and Wistar-lmamichi strain non-pregnant and pregnant female rats were orally given aqueous solution of CHA. Acute toxic symptoms caused by CHA were staggering gait, salivation, lacrymation and convulsion. In postmortem examination, remarkable hemorrhage in lung, marked edematous change in the mucous membrane of fore-stomach and congestion in glandural-stomach and duodenum were observed.Median lethal doses calculated following oral administration of CHA were 236.6mg/kg, 278.2mg/kg for Wistar strain female and male rats, 155.7mg/kg for Wistar-Imamichi strain female rats and 180.1mg/kg for pregnant Wistar-Imamichi rats, respectively.Pregnant Wistar-Imamichi strain rats were orally given once daily for 7 days from day 7 to 13 of gestation at the doses of 1.8, 3.6, 18 and 36mg/kg of CHA and its sulfate of 71.6mg/kg.In the highest dose of CHA, a suppression in body weight gain and reduction in food and water consumptions were ebserved and 2 of 17 dams died following the administration. No significant difference between the treated and control groups was found in maintenance of pregnancy and in fetal development, and no abnormal ities of the fetuses such as resorption and malformation were observed in all groups.