Abstract
The composition of the α-branched-β-hydroxylated fatty acids from Corynebacterium ovis was studied. These acids were fractionated by argentation thin-layer chromatography in three species according to the degree of unsaturation of their O-acetylated methyl ester derivatives; the isolated fractions were analysed by gas chromatography combined with mass spectrometry. Corynomycolic (2-tetradecyl-3-hydroxy octadecanoic), corynomycolenic (2-tetradecyl-3-hydroxy-11-octadecenoic) and corynomycoldienic (2-tetradec-7′-enyl-3-hydroxy-11-octadecenoic) acids were identified; these acids represented the main component of the saturated, mono- and di-unsaturated species, respectively. On the other hand, a short-chain corynomycolic acid (2-tetradecyl-3-hydroxy decanoic acid), C 24H 48O 3 was found.
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