Abstract
It has been well known that differential centrifugation by the use of ultracentrifuge is the most effective method for the purification of animal viruses. However, as the use of ultracentrifuge is so far fairly limited in this country, other methods for partially purifying viruses should be investigated more in detail. Adsorption and elution of viruses by means of adsorbents have been attempted by numerous workers for the partial purification of viruses. As adsorbents, resins (1) (2), clay (3), kaolin (4) (5) (6) as well as chicken red blood cells (7) (8) (9), in the case of viruses having hemagglutinins, have been employed. In recent years it has been shown that methyl alcohol at low temperature precipitates various proteins as well as viruses without denature or inactivation. And this fact has found wide application in the purification of various viruses (10) (11) (12) (13). The present work deals with the purification of influenza virus by methods in which adsorbents and precipitants are used or combined.
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