Abstract
Partially purified fractions of the water-soluble extract of Entamoeba histolytica (DKB strain) obtained by chromatography in Sephadex G-200 (F1, F2, and F4) and G-100 (F3a, F3b, F3c, and F3d) gels were used in various serological tests and their reactivities compared with the whole 1:40 antigen. The main haemagglutinating (HA) and complement-fixing (CF) activities were confined to fractions F1 and F2, which had molecular weights of 650 000 and 229 000, respectively. The 4 to 6 μg/ml of protein contained in these fractions at the optimum dilution gave antibody liters comparable to those for the whole antigen, which had about 77 μg/ml of protein. The other antigen fractions (F4, F3a, F3b, F3c, and F3d) showed very little activity. Fraction F1 had two main precipitin bands (1 and 2), which showed reaction of identity with two of the bands in fraction F2. The other fractions which showed some HA and CF reactivity had trace amounts of these antigens. It is presumed that the antigens specific for precipitin bands 1 and 2 are the main CF and HA antigens.
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