Abstract
Abstract Studies of the transformations of substituted urea herbicides in rumen fluid, using metobromuron as a pilot compound, indicated that one of the products is the corresponding aniline. Because it was possible for a diazotization reaction to take place from the reduction of nitrate to hydroxylamine and ammonia through a transient nitrite step, the formation of azo compounds in rumen fluid was investigated. Incubation of 3,4-dichloroaniline with rumen fluid did not result in t he formation of any measurable azo compounds. The stability in rumen fluid of a known azo compound, 3,3’,4,4’-tetrachloroazobenzene, was also studied. Evidence is presented to show that azo compounds can break down in rumen fluid.
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