Abstract

After an incubation of rat intestinal slices in vitro in a solution of L-phenylalanine in sodium-free, potassium-substituted buffer, even of long duration, the mean tissue concentration of the substrate never attains the level of the surrounding medium. The amino-acid uptake under such conditions kinetically resembles free diffusion, and is directly proportional to the concentration of the substrate in the incubation medium.

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