Abstract
Assays for African swine fever virus (ASFV) were conducted on 16 tissues of pigs serially killed at daily intervals following intranasal inoculation of ASFV. Virus was first detected at 1 day postinoculation in the tonsils, mandibular lymph nodes, and in circulating leukocytes in the blood. Once virus appeared in blood, it spread rapidly throughout the body and was detected at 3 days in all tissues examined. Highest viral titers were observed in tissues containing a large component of reticulo-endothelial cellular elements (108 50% hemadsorption units per gram). This correlates favorably with the observation that the significant lesions of African swine fever occur in such tissues.
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