STUDIES ON THE NUCLEIC ACID OF CORTI'S ORGAN

Abstract

The author obtained corti's organ of guinea pigs and observed each cell-group from tangential direction using Feulgen reaction and M.G.P. stains.The results were as follows:-1st. Chapter1) 2% Formalin was the most favorable for the fixation of corti's organ.2) Chromatin granules in cell nuclei showed three formulas of metabolic phase.3) Concerning size of outer hair cells, the 1st raw was smaller than the 2nd and the 3rd raw, and was easily injured.4) Deiter's and Hensen's cells showed relative resistance to physical and chemical stimuli.2nd Chapter1) By D.H.S.M. injection, corti's organ showed functional disorder in the early stage. When the dosage was increased, the damage of the cells and injuries of head plate was observed.The changes were remarkable in the 1st and the 2nd winding.2) When Vitamin B1 was injected togather, these changes were less remarkable and recovered more rapidly.3) Deiter's cells were More resistant to the high dose of D.H.S.M., but once the functional dis order developed, recovery was slower than the hair cells. This might be one of the factor in the delay of the recovery the auditory disorders caused by D.H.S.M.3rd Chapter1) Sound stimulation produced more apparent changes in the 1st raw of outer hair cells than the 2nd and the 3rd raw of the cells.2) Stimulation by impulse tone produced the changes in outer hair cells about 2 min. after the stimulation but recovered within 2_??_4 hours.3) By investigation of the cell metabolism in camera silenta, outer hair cells were thought to show restitution.phase in about 2 hours and extrusion-phase in 8 hours.4) Thus first 4_??_8 hours after the stimutation are most important for the recovery from early injuries by sound stimulation, and during this period, release from sound stimuli and medication are recommended.