Abstract
Summary The neutralization of Japanese encephalitis virus by rabbit antiserum was studied by the plaque assay method in porcine kidney stable cell cultures. Neutralizing antibodies in the antiserum were significantly potentiated in the presence of normal fresh guinea pig serum. The kinetic neutralization at pH 8.0 in the presence of fresh guinea pig serum followed the first order kinetics within a certain limited incubation period. The slope of the curve was proportional to the serum concentration and independent of the initial virus concentration. The neutralization rate constant (K value) calculated from each curve was found to be applicable to the antigenic characterization of the virus as a precise measure of neutralizing potency of antiserum against a given virus strain. In reciprocal neutralization each serum neutralized the isologous virus more rapidly than any other strains. The antigenic relationships among strains isolated from the field were examined by this method.
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