Abstract
In this paper, Sitophilus oryzae, Sitophilus zeamais, and Sitophilus granarius, three economically significant grain pests are thoroughly analyzed in terms of their morphological and molecular differences. The work provides critical insights into species identification, genetic differentiation, and adaptation by contrasting conventional morphological features and genetic approaches. The samples were collected from eight talukas of district Khairpur: Kingri, Kot Deji, Khairpur, Gamabat, Mirwah, Sobhodero, Nara, and Faizganj during the year 2018-2022. The samples were identified on the basis of morphology and extraction of DNA was done. The samples were sent for sequencing. The findings provide important evolutionary insights and aid in pest management. Additionally, the text describes the distinguishing physical characteristics of mature Sitophilus granarius, maize weevils, and rice weevils, emphasising size, colour, markings, and anatomical aspects. Understanding the differences among various weevil species is made easier by this information.
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